Journal: Scientific Reports

Article Title: Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism

doi: 10.1038/s41598-025-87246-7

Figure Lengend Snippet: Regulation of DHRS4-AS1 on OS cell proliferation and apoptosis in vitro. (A) Differential expression of DHRS4-AS1 in human osteoblast cell line and three OS cell lines, *Represent comparison with the hFOB1.19 group. ( B) Overexpression expression of DHRS4-AS1 in MG63, *Represent comparison with the BC group. ( C) DHRS4-AS1 silencing models in 143B cells, *Represent comparison with the BC group. ( D) CCK-8 results showing proliferation level over 4 consecutive days for MG63 cells in each group, *Represent comparison with the NC group. ( E) Proliferation level for 143B cells in each group, *Represent comparison with the NC group. ( F) Tunel results showing apoptosis level for MG63 cells in each group. ( G) Tunel results showing apoptosis level for 143B cells in each group. ( H) Quantitative analysis of apoptosis rate of MG63 cells in different groups, *Represent comparison with the NC group. ( I) Quantitative analysis of apoptosis rate of 143B cells in different groups, *Represent comparison with the NC group. ( J) Apoptosis-related protein levels of MG63 and 143B cells in each group were determined using western blot. ( K) Quantitative analysis of apoptosis-related protein in MG63 cells, *Represent comparison with the NC group. ( L) Quantitative analysis of apoptosis-related protein in 143B cells, *Represent comparison with the NC group. * p <0.05, ** p <0.01, *** p <0.001, the scale is 100 μm. BC and NC represent Blank Control and Negative Control respectively.

Article Snippet: The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231). hFOB1.19 cells were cultured in DMEM/F12 (SH30126.01; HyClone; Cytiva) containing 10% FBS (10099; Thermo Fisher Scientific, Inc.) and 0.3 mg/ml G418 (PB180125; Procell Life Science & Technology Co., Ltd.) with 5% CO 2 at 37 ̊C.

Techniques: In Vitro, Quantitative Proteomics, Comparison, Over Expression, Expressing, CCK-8 Assay, TUNEL Assay, Western Blot, Control, Negative Control

Journal: Scientific Reports

Article Title: Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism

doi: 10.1038/s41598-025-87246-7

Figure Lengend Snippet: DHRS4-AS1 directly interacts with miR-362-5p and represses its expression. ( A) The expression level of miR-362-5p in osteosarcoma cell lines and the human osteoblast cell line, *Represent comparison with the Hfob1.19 group. ( B) The expression level of miR-362-5p in MG63 cells was increased after transfection with miR-362-5p mimic, *Represent comparison with the mm group. ( C) After co-transfection with DHRS4-AS1 and miR-362-5p mimic, DHRS4-AS1 reversed the high expression of miR-362-5p in MG63 cells, *Represent comparison with the mNC group, # represents comparison with the mm group. ( D-E) Expression levels of apoptosis-related proteins in MG63 cells, *Represent comparison with the mNC group, # represents comparison with the mm group. ( F) cck8 assay was performed to detect the proliferation and apoptosis levels of MG63 cells after co-transfection with DHRS4-AS1 and miR-362-5p mimic, *Represent comparison with the mNC group, # represents comparison with the mm group. ( G) Quantification of apoptosis rate in MG63 cells after co-transfection with DHRS4-AS1 and miR-362-5p mimic, *Represent comparison with the mNC group, # represents comparison with the mm group. ( H) Tunel detection of apoptosis in MG63 cells after co-transfection with DHRS4-AS1 and miR-362-5p mimic. * p <0.05, ** p <0.01, *** p <0.001, ### p <0.001, the scale is 100 μm. mm represent miR-362-5p mimic group. mNC represent the mimic Negative Control group. D + mm represents the DHRS4-AS1 + miR-362-5p mimic group, indicating that both DHRS4-AS1 and miR-362-5p are overexpressed. D + mNC represents the DHRS4-AS1 + mimic Negative Control group, indicating that DHRS4-AS is overexpressed but not expressing miR-362-5p. BC represents Blank Control group.

Article Snippet: The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231). hFOB1.19 cells were cultured in DMEM/F12 (SH30126.01; HyClone; Cytiva) containing 10% FBS (10099; Thermo Fisher Scientific, Inc.) and 0.3 mg/ml G418 (PB180125; Procell Life Science & Technology Co., Ltd.) with 5% CO 2 at 37 ̊C.

Techniques: Expressing, Comparison, Transfection, Cotransfection, CCK-8 Assay, TUNEL Assay, Negative Control, Control

Journal: Scientific Reports

Article Title: Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism

doi: 10.1038/s41598-025-87246-7

Figure Lengend Snippet: MiR-362-5p mediates the proliferation and apoptosis of OS cells through NPEPPS. (A-B) The expression level of NPEPPS in osteosarcoma cell lines and the human osteoblast cell line, *Represent comparison with the Hfob1.19 group. ( C-D) NPEPPS was overexpressed in MG63 OS cells, *Represent comparison with the pN group. ( E-F) the expression level of NPEPPS and poptosis related protein in MG63 cells after cotransfected miR-362-5p mimic and NPEPPS, *Represent comparison with the NC group. ( G) The Tunel assay was used to successfully detect apoptosis in MG63 cells after co-transfection with miR-362-5p mimetics and NPEPPS. ( H) The CCK-8 assay was employed to determine the proliferation and apoptosis levels of MG63 cells co-transfected with miR-362-5p mimetics and NPEPPS, *Represent comparison with the NC group. ( I) The apoptosis rate in MG63 cells co-transfected with miR-362-5p mimic and NPEPPS was quantified, *Represent comparison with the NC group, # represents comparison with the pN group. * p <0.05, ** p <0.01, *** p <0.001, # p <0.01, ## p <0.001, ### p <0.001, the scale is 100 μm. NC represents Negative Control group. BC represents Blank Control group. mm represent miR-362-5p mimic group. pN represents the pCMV NPEPPS group, which overexpresses NPEPPS using the pCMV NPEPPS vector. mm + pN represents the miR-362-5p mimetic + pCMV NPEPP group, indicating the overexpression of both miR-362-5p and NPEPPS using miR-362-5p mimetic transfection and pCMV NPEP vector.

Article Snippet: The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231). hFOB1.19 cells were cultured in DMEM/F12 (SH30126.01; HyClone; Cytiva) containing 10% FBS (10099; Thermo Fisher Scientific, Inc.) and 0.3 mg/ml G418 (PB180125; Procell Life Science & Technology Co., Ltd.) with 5% CO 2 at 37 ̊C.

Techniques: Expressing, Comparison, TUNEL Assay, Cotransfection, CCK-8 Assay, Transfection, Negative Control, Control, Plasmid Preparation, Over Expression